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murine endothelial cell line svec4 10  (ATCC)


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    ATCC murine endothelial cell line svec4 10
    Murine Endothelial Cell Line Svec4 10, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+endothelial+cell+line+svec4+10/SVEC4-10/pmc10766952-345-1-10
    Average 96 stars, based on 382 article reviews
    murine endothelial cell line svec4 10 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Human Neutrophil Peptide-1 Inhibits Thrombus Formation Under Arterial Flow via Its Terminal Free Cysteine Thiols
    Article Snippet: Aliphatic HNP-1 was synthesized by replacing all cysteine residues in HNP-1 with an alanine plus N-terminal acetylation and C-terminal amidation (Biomatik, Wilmington, DE). .. The transformed murine endothelial cell line SVEC4–10 was purchased from ATCC (Manassas, VA) [ 15 , 16 ]. .. Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum, penicillin, and streptomycin were purchased from Life Technology (Grand Island, NY).

    Cell Culture:

    Article Title: Magnetic targeting of adoptively transferred tumour-specific nanoparticle-loaded CD8 + T cells does not improve their tumour infiltration in a mouse model of cancer but promotes the retention of these cells in tumour-draining lymph nodes
    Article Snippet: .. The murine endothelial cell line SVEC4-10 (ATCC: CRL-2181) was cultured in DMEM with 10% FBS, 2 mM l -glutamine, 1 mM sodium pyruvate, and 100 U/ml penicillin/streptomycin (P/S) under standard culture conditions (37 °C, 5% CO 2 , 90% relative humidity). .. The tumour-cell line EG7-OVA (ATCC: CRL2113) was cultured in RPMI1640 with 10% FBS, 2 mM l -glutamine, 1 mM sodium pyruvate, 50 μM 2-mercaptoethanol, 10 mM HEPES, non-essential amino acids 1X, 100 U/ml P/S (complete RPMI medium), and in the presence of 0.4 mg/ml geneticin (G418, ThermoFisher Scientific) to select only plasmid-carrying cells, all under standard culture conditions.

    Article Title: The Peptide/Antibody-Based Surface Decoration of Calcium Phosphate Nanoparticles Carrying siRNA Influences the p65 NF-κB Protein Expression in Inflamed Cells In Vitro
    Article Snippet: The murine monocyte cell line J774A.1 (CLS Cell Lines Service GmbH, Eppelheim, Germany) was cultured in DMEM/F-12 (1 + 1) (+)-L-glutamine (Gibco, Germany) supplemented with 10 vol% fetal calf serum (FCS; Gibco). .. The murine endothelial cell line SVEC4-10 (American Type Culture Collection, ATCC, 141 CCL81, Rockville, MD) was cultured in DMEM (1x) + GlutaMAX TM -I (Gibco, Germany) supplemented with 10 vol% heat-inactivated FCS. .. The murine T-lymphocyte (lymphoma) cell line TK-1 (ATCC) was cultured in RPMI 1640 medium supplemented with 10 vol% FCS, 0.1 mM non-essential amino acids, and 0.05 mM 2-mercaptoethanol (Thermo Fisher, Germany).

    Article Title: Magnetic Nanoparticles Attached to the NK Cell Surface for Tumor Targeting in Adoptive Transfer Therapies Does Not Affect Cellular Effector Functions
    Article Snippet: .. The murine endothelial cell line SVEC4-10 (ATCC: CRL-2181) was cultured in DMEM with 10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, and 100 U/ml P/S. ..



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    murine endothelial cell line svec4 10  (Procell Inc)
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    Procell Inc murine endothelial cell line svec4 10
    A Subcutaneous tumor growth in mice co-injected with CRC cells and either Egr1⁺ or control neutrophils. Tumor volume was monitored for 21 days. Inset shows representative tumors at endpoint ( n = 5 mice, two-way ANOVA). B CD31 immunofluorescence (green) and vascular density quantification in subcutaneous tumors. Yellow arrowheads indicate endothelial structures ( n = 5 mice, Student’s t test). Scale bars: 100 µm. C mIHC of pre-metastatic livers (day 14) showing spatial association of Egr1 + neutrophils (white arrowheads) and CD31⁺ vessels (yellow arrowheads). Scale bars: 100 µm. D , E Co-localization analysis of Egr1⁺ neutrophils and CD31⁺ vasculature in mouse ( D , n = 2 mice) and human ( E , n = 2 patients) CRLM samples, highlighting the tumor boundary. Scale bars: 100 µm. F , <t>I</t> <t>SVEC4-10</t> endothelial cell viability (CCK-8) ( n = 3 independent experiments, Student’s t test). G , J Endothelial functional assays using supernatants from triple co-cultures treated with Rog or WRW4 ( n = 3 independent experiments, Student’s t test). H , K Endothelial migration (scratch wound assay) ( n = 3 independent experiments, Student’s t test). L Representative tube formation images (HUVEC and SVEC4-10) treated with neutrophil-conditioned media ( n = 3 independent experiments). Scale bars: 20 µm. M , N Quantification of vessel numbers ( M ) and junction points ( N ) ( n = 3 independent experiments, Student’s t test). Where applicable, all statistical tests are two-sided. Data are presented as mean ± s.d. Source data and exact p values are provided as a Source Data file.
    Murine Endothelial Cell Line Svec4 10, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    murine endothelial cell line svec4 10  (ATCC)
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    ATCC murine endothelial cell line svec4 10
    A Subcutaneous tumor growth in mice co-injected with CRC cells and either Egr1⁺ or control neutrophils. Tumor volume was monitored for 21 days. Inset shows representative tumors at endpoint ( n = 5 mice, two-way ANOVA). B CD31 immunofluorescence (green) and vascular density quantification in subcutaneous tumors. Yellow arrowheads indicate endothelial structures ( n = 5 mice, Student’s t test). Scale bars: 100 µm. C mIHC of pre-metastatic livers (day 14) showing spatial association of Egr1 + neutrophils (white arrowheads) and CD31⁺ vessels (yellow arrowheads). Scale bars: 100 µm. D , E Co-localization analysis of Egr1⁺ neutrophils and CD31⁺ vasculature in mouse ( D , n = 2 mice) and human ( E , n = 2 patients) CRLM samples, highlighting the tumor boundary. Scale bars: 100 µm. F , <t>I</t> <t>SVEC4-10</t> endothelial cell viability (CCK-8) ( n = 3 independent experiments, Student’s t test). G , J Endothelial functional assays using supernatants from triple co-cultures treated with Rog or WRW4 ( n = 3 independent experiments, Student’s t test). H , K Endothelial migration (scratch wound assay) ( n = 3 independent experiments, Student’s t test). L Representative tube formation images (HUVEC and SVEC4-10) treated with neutrophil-conditioned media ( n = 3 independent experiments). Scale bars: 20 µm. M , N Quantification of vessel numbers ( M ) and junction points ( N ) ( n = 3 independent experiments, Student’s t test). Where applicable, all statistical tests are two-sided. Data are presented as mean ± s.d. Source data and exact p values are provided as a Source Data file.
    Murine Endothelial Cell Line Svec4 10, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    murine lymphatic endothelial cell line  (ATCC)
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    Fig. 2. Functional assessment of Az-EVs in 2D <t>endothelial</t> culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
    Murine Lymphatic Endothelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    murine endothelial cell line svec410  (ATCC)
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    Fig. 2. Functional assessment of Az-EVs in 2D <t>endothelial</t> culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
    Murine Endothelial Cell Line Svec410, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    murine endothelial cell lines  (ATCC)
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    Fig. 2. Functional assessment of Az-EVs in 2D <t>endothelial</t> culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
    Murine Endothelial Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell lines murine endothelial svec4 10 atcc cat  (ATCC)
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    ATCC cell lines murine endothelial svec4 10 atcc cat
    Fig. 2. Functional assessment of Az-EVs in 2D <t>endothelial</t> culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
    Cell Lines Murine Endothelial Svec4 10 Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+endothelial+cell+line+svec4+10/SVEC4-10/pm29861387-247-78-83
    Average 96 stars, based on 1 article reviews
    cell lines murine endothelial svec4 10 atcc cat - by Bioz Stars, 2026-10
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    Image Search Results


    A Subcutaneous tumor growth in mice co-injected with CRC cells and either Egr1⁺ or control neutrophils. Tumor volume was monitored for 21 days. Inset shows representative tumors at endpoint ( n = 5 mice, two-way ANOVA). B CD31 immunofluorescence (green) and vascular density quantification in subcutaneous tumors. Yellow arrowheads indicate endothelial structures ( n = 5 mice, Student’s t test). Scale bars: 100 µm. C mIHC of pre-metastatic livers (day 14) showing spatial association of Egr1 + neutrophils (white arrowheads) and CD31⁺ vessels (yellow arrowheads). Scale bars: 100 µm. D , E Co-localization analysis of Egr1⁺ neutrophils and CD31⁺ vasculature in mouse ( D , n = 2 mice) and human ( E , n = 2 patients) CRLM samples, highlighting the tumor boundary. Scale bars: 100 µm. F , I SVEC4-10 endothelial cell viability (CCK-8) ( n = 3 independent experiments, Student’s t test). G , J Endothelial functional assays using supernatants from triple co-cultures treated with Rog or WRW4 ( n = 3 independent experiments, Student’s t test). H , K Endothelial migration (scratch wound assay) ( n = 3 independent experiments, Student’s t test). L Representative tube formation images (HUVEC and SVEC4-10) treated with neutrophil-conditioned media ( n = 3 independent experiments). Scale bars: 20 µm. M , N Quantification of vessel numbers ( M ) and junction points ( N ) ( n = 3 independent experiments, Student’s t test). Where applicable, all statistical tests are two-sided. Data are presented as mean ± s.d. Source data and exact p values are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Hepatocytes functionally reprogrammed by KIAA1199-high colorectal cancer cells favour the accumulation of pro-metastatic Egr1 + neutrophils

    doi: 10.1038/s41467-026-69250-1

    Figure Lengend Snippet: A Subcutaneous tumor growth in mice co-injected with CRC cells and either Egr1⁺ or control neutrophils. Tumor volume was monitored for 21 days. Inset shows representative tumors at endpoint ( n = 5 mice, two-way ANOVA). B CD31 immunofluorescence (green) and vascular density quantification in subcutaneous tumors. Yellow arrowheads indicate endothelial structures ( n = 5 mice, Student’s t test). Scale bars: 100 µm. C mIHC of pre-metastatic livers (day 14) showing spatial association of Egr1 + neutrophils (white arrowheads) and CD31⁺ vessels (yellow arrowheads). Scale bars: 100 µm. D , E Co-localization analysis of Egr1⁺ neutrophils and CD31⁺ vasculature in mouse ( D , n = 2 mice) and human ( E , n = 2 patients) CRLM samples, highlighting the tumor boundary. Scale bars: 100 µm. F , I SVEC4-10 endothelial cell viability (CCK-8) ( n = 3 independent experiments, Student’s t test). G , J Endothelial functional assays using supernatants from triple co-cultures treated with Rog or WRW4 ( n = 3 independent experiments, Student’s t test). H , K Endothelial migration (scratch wound assay) ( n = 3 independent experiments, Student’s t test). L Representative tube formation images (HUVEC and SVEC4-10) treated with neutrophil-conditioned media ( n = 3 independent experiments). Scale bars: 20 µm. M , N Quantification of vessel numbers ( M ) and junction points ( N ) ( n = 3 independent experiments, Student’s t test). Where applicable, all statistical tests are two-sided. Data are presented as mean ± s.d. Source data and exact p values are provided as a Source Data file.

    Article Snippet: Murine colorectal cancer cell lines MC38 (Cat# CL-0972) and CT26 (Cat# CL-0071), murine hepatocyte line AML12 (Cat# CL-0602), murine endothelial cell line SVEC4-10 (Cat# CL-0221), human umbilical vein endothelial cells (HUVEC, Cat# CL-0675), and human myeloid cell line HL-60 (Cat# CL-0110) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

    Techniques: Injection, Control, Immunofluorescence, CCK-8 Assay, Functional Assay, Migration, Scratch Wound Assay Assay

    Fig. 2. Functional assessment of Az-EVs in 2D endothelial culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Journal of dairy science

    Article Title: Chemical interactions among caseins during rennet coagulation of milk.

    doi: 10.3168/jds.2021-21071

    Figure Lengend Snippet: Fig. 2. Functional assessment of Az-EVs in 2D endothelial culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Murine lymphatic endothelial cell line (SVEC4-10, CRL-2181 ATCC) were grown and maintained in high (4.5 g/L) glucose containing Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher) supplemented with 10% heat-inactivated exosome-depleted fetal bovine serum (FBS; Thermo Fisher).

    Techniques: Functional Assay, Immunofluorescence, Staining, Labeling, Imaging, Tube Formation Assay, Wound Healing Assay, Migration, Positive Control

    Fig. 5. Assessing host vascular ingrowth and macrophage recruitment in EV-functionalized collagen implants. (A,B) Histological analysis (A) and quanti fication (B) of host vascular ingrowth via endothelial CD31 staining (n = 7). (C,D) Histological analysis (C) and quantification (D) of M1 macrophage staining (CCR7) (n = 7). (E,F) Histological analysis (E) and quantification (F) of M2 macrophage staining (MMR) (n = 7). (G,H) IVIS images (G) and the quantification of fluorescence intensities (H) of the lectin accumulation in the implants 20 min after i.v. injection (n = 4). (I) Representative images for lectin perfused vasculature in the implants. One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Journal of dairy science

    Article Title: Chemical interactions among caseins during rennet coagulation of milk.

    doi: 10.3168/jds.2021-21071

    Figure Lengend Snippet: Fig. 5. Assessing host vascular ingrowth and macrophage recruitment in EV-functionalized collagen implants. (A,B) Histological analysis (A) and quanti fication (B) of host vascular ingrowth via endothelial CD31 staining (n = 7). (C,D) Histological analysis (C) and quantification (D) of M1 macrophage staining (CCR7) (n = 7). (E,F) Histological analysis (E) and quantification (F) of M2 macrophage staining (MMR) (n = 7). (G,H) IVIS images (G) and the quantification of fluorescence intensities (H) of the lectin accumulation in the implants 20 min after i.v. injection (n = 4). (I) Representative images for lectin perfused vasculature in the implants. One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Murine lymphatic endothelial cell line (SVEC4-10, CRL-2181 ATCC) were grown and maintained in high (4.5 g/L) glucose containing Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher) supplemented with 10% heat-inactivated exosome-depleted fetal bovine serum (FBS; Thermo Fisher).

    Techniques: Staining, Fluorescence, Injection